Redox Biology has published a paper written by the students and staff of JU Faculty of Biochemistry, Biophysics and Biotechnology, JU Faculty of chemistry and JU Małopolska Centre of Biotechnology. The paper is devoted to the issue of changes in the ‘paramagnetome’ (i.e. the population of paramagnetic centres) in the livers of mice.
The article is the result of successful collaboration between experts from various biological and chemical fields, hence the diversity of methods and approaches used in the experimental part of the study. The aim of the project was to showcase the various biological aspects of ischemic necrosis using a sterile necrosis model developed at the Faculty of Biology, Biophysics and Biotechnology.
Since researchers expected changes to mostly occur in the proportions and components of liver free radicals, they focused primarily on EPR spectroscopy. Other experiments discussed in the paper featured (immuno)histochemical marking and digital image analysis.
Publication costs were covered by the Polish Ministry of Science and Higher Education within the framework of Elsevier open programme pilot scheme. The full paper, entitled Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver, is available here.
